There in lies the puzzle...Now this is going to be kool!
Just thinking out loud here, but since the bike wheels will likely spin faster than the table top Zoetropev or the turntable; will you space the images on your animation reel farther apart?
There are certain fundamental elements of a zoetrope that will make this detail somewhat of an 'Easter Egg'. Usually, you need to be able to isolate the images frame by frame. This is done by looking through a slot in the carousel (on a traditional zoetrope).
With the record version you can use a strobe light, or view it through your camera at 'x' shutterspeed. There is even a multifaceted mirror you can place over the center pin, that reflects the image one frame at a time while the record is playing.
So to answer your question... the spacing can relate to speed, but also to detail. I might have to actually use more images, that are CLOSER together, as a result of more frames per second. Less images (spread farther apart) may result in the animation being choppy, or happening too quickly to appreciate.
I am trying to extrapolate, from a 12" record to a 20" hubcap, how many images to use. I will draw out something simple and tape it to the disc to see how/if it works. I might even try and create a slot for isolation on the frame side of the dropouts so it can be seen with or without a strobe/camera.
@OddJob here's an example of the record playing in the video (12" @ 33 1/3rpm)
On further reflection, my points about the animation being choppy or it happening too fast to appreciate, would only apply in one application- if I make the animation go from center to outer edge, centrifugally. If I only do a band around the edge (or a series of parallel bands), then putting the images farther apart would make it repeat faster yet uninterrupted.